Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries.
نویسندگان
چکیده
A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 70 12 شماره
صفحات -
تاریخ انتشار 2004